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KMID : 0364820090450030275
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Korean Journal of Microbiology
2009 Volume.45 No. 3 p.275 ~ p.280
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Production and Structural Analysis of Cellulose by Acetobacter sp. V6 Using Static Culture
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Kim Jeong-Do
Jung Ho-Il
Jeong Jin-Ha
Park Ki-Hyun
Jeon Young-Dong
Hwang Dae-Yean
Lee Chung-Yeol
Son Hong-Joo
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Abstract
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The optimal medium compositions for the production of bacterial cellulose (BC) by a Acetobacter sp. V6, which was isolated from the traditionally fermented vinegar in Korea, were investigated in static cultures. The optimum medium compositions for BC production were 3% glucose, 3% soytone, 0.8% K2HPO4, and 0.4% ethanol, respectively. Adding NaH2PO4 or KH2PO4 had not shown the increase in BC production. Under the optimum medium compositions, the highest BC production was 44.67 g/m2 in 8 days and the thickness of BC pellicle was about 1 cm. Structural properties of BC produced in the optimal medium were studied using Fourier-transform infrared spectroscopy and X-ray diffractometer. BC from the optimal medium was found to be of cellulose type I, the same as typical native cellulose. No difference in the compositions between bacterial and plant celluloses, but BC showed unique micro-network structure and high crystallinity (82%).
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KEYWORD
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Acetobacter sp., bacterial cellulose, cellulose type I, static culture
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